Saturday, 20 December 2014

Practical 4 - Uniformity of weight of tablets and capsules.

Objective:

To determine the uniformity of weight of tablets and capsules.


Introduction:

            Uniformity of Content is a pharmaceutical analysis technique for the quality control of capsules or tablets. Multiple capsules or tablets are selected at random and a suitable analytical method is applied to assay the individual content of the active ingredient in each capsule or tablet. In weight variation test, measurement of contents is done by estimation of contents based on weight. Pharmacists often use and misuse this WV test by doing this test on all dosage units. If it is used correctly, this WV test can be used to measure content uniformity (CU). There are some conditions in which the weight difference can determine the percentage difference in the API in the individual dosage units. Hence this WV test can be useful in the quality control of drug production. This is an important test in technology of pharmaceutical.


Apparatus and materials:

Capsules , tablets , Weighing boats,   Weigh balance


Procedures:

Tablets: Paralgin tablet 500mg
1. 20 tablets were selected randomly and weighed. The average weight was determined.
2. The tablets were weighed individually and for each tablet, the percentage deviation of its weight         from the average weight was determined.
3. The deviation of individual weight from the average weight should not exceed the limits given           below.
Average weight of tablet
Deviation (%)
Number of tablets
Less than 80 mg
±10.0
±20.0
Minimum 18
Maximum 2
80 mg to 250 mg
±7.5
±15.0
Minimum 18
Maximum 2
More than 250 mg
±5.0
±10.0
Minimum 18
Maximum 2



Capsules: Ampilin antibiotic capsule 250mg
1. 20 capsules were selected at random.
2. One capsule was weighed. The capsule was then opened and the contents were removed as                 completely as possible. The emptied shells were weighed. The net weight of its contents was               determined, that is by subtracting the weight of the shells from the weight of the intact capsule.
3. The procedure was repeated with the other 19 capsules.
4. The average net weight was determined from the sum of the individual net weights.
5. The percentage deviation was determined from the average net weight for each capsule. The               deviation of individual net weight should not exceed the limits given below.

Average net weight of capsule
Deviation (%)
Number of tablets
Less than 300 mg
±10.0
±20.0
Minimum 18
Maximum 2
300 mg or more
±7.5
±15.0
Minimum 18
Maximum 2





Results And Calculations:

Tablets
No.
Individual weight of tablets (g)
Percentage of deviation (%)
1.
0.6740
-0.43
2.
0.7038
+3.97
3.
0.6890
+1.79
4.
0.6762
-0.10
5.
0.6820
+0.75
6.
0.6656
-1.67
7.
0.6570
-2.94
8.
0.6726
-0.64
9.
0.6868
+1.46
10.
0.6808
+0.58
11.
0.6853
+1.24
12.
0.6811
+0.62
13.
0.6796
+0.40
14.
0.6712
-0.84
15.
0.6806
+0.58
16.
0.6611
-2.33
17.
0.6573
-2.90
18.
0.6770
+0.15
19.
0.6884
+1.70
20.
0.6684
-1.26


Results:
Total weight of 20 tablets = 13.5380 g
Average weight =  13.5380g/20
                           = 0.6769 g

**Percentage of deviation = [ (𝑖𝑛𝑑𝑖𝑣𝑖𝑑𝑢𝑎𝑙 𝑤𝑒𝑖𝑔ℎ𝑡)/(𝑎𝑣𝑒𝑟𝑎𝑔𝑒 𝑤𝑒𝑖𝑔ℎ𝑡)  x 100] -100

Capsules

No.
Individual weight of capsules (g)
Weight of emptied shells (g)
Net weight of the contents (g)
Percentage deviation (%)
1.
0.3510
0.0606
0.2904
+3.74
2.
0.3537
0.0641
0.2896
+3.47
3.
0.3543
0.0629
0.2914
+4.11
4.
0.3646
0.0625
0.3021
+7.93
5.
0.3531
0.0590
0.2941
+5.07
6.
0.3592
0.0614
0.2978
+6.40
7.
0.3631
0.0631
0.3000
+7.18
8.
0.3570
0.0631
0.2879
+2.86
9.
0.3628
0.0675
0.2953
+5.50
10.
0.3516
0.0617
0.2899
+3.57
11.
0.3667
0.0654
0.3013
+7.65
12.
0.3393
0.0617
0.2776
-0.82
13.
0.3596
0.0624
0.2972
+6.18
14.
0.3479
0.0625
0.2854
+1.97
15.
0.3642
0.0590
0.3052
+9.04
16.
0.3504
0.0630
0.2874
+2.68
17.
0.3652
0.0619
0.2973
+6.22
18.
0.3520
0.0618
0.2902
+3.68
19.
0.3630
0.0613
0.3017
+7.79
20.
0.3595
0.0648
0.2947
+5.29

Total net weight of the content of 20 capsules = 5.5989 g
Average net weight =  5.5989/20
                                 = 0.2800 g


Discussion:

For tablets, since our average weight is 676.9 mg, the deviation of individual net weight should not exceed the limits given below:
More than 250 mg
±5.0
±10.0
Minimum 18
Maximum 2

From the results, we can see that the all the tablets are having standard deviation of ± 5.0. This is shows that 20 tablets having this type of deviation. This may indicate the uniformity of the capsules production.
However, the variation in the weight of the tablets may be explained by the following. Factors such as the flowing properties of the powder, the speed of tableting machine, the pressure used in compression and the type of machines used in tableting may affect the weight of a tablet. However the two most common causes of weight variation are the differences in the bulk densities and particle size distribution during compression

For capsules, since our average weight is 280.0 mg, the deviation of individual net weight should not exceed the limits given below:
Less than 300 mg
±10.0
±20.0
Minimum 18
Maximum 2

From the results, all our capsules are having a deviation of ± 10%, which means that all capsules have uniform weights. This may indicate the uniformity of the capsules production. At the same time, this uniformity may also be caused by the usage of a good weighing balance. The weighing balance used for measuring the weight of capsule is more accurate as it is a more advanced balance.
If the weight variation in capsule exceeds the limit, this may be due to the defect of the capsule filling machine. The machine may have misalignment of the upper and lower capsule segments or problem in filling the capsule with the target fill weight.



Conclusion:

Both tablets and capsules tested have passed the test of weight uniformity. This uniformity may also be caused by the usage of a good weighing balance. The weighing balance used for measuring the weight of capsule is more accurate as it is a more advanced balance.



References:

2. Donald K. Lightfoot. (n.d.) Answers To 10 Common Questions About Capsule Filling. Retrieved date. 21st December 2013. From http://capsugel.com/media/library/answers-to-10-common-questions-about-capsule-filling.pdf
3. Dshravani. (2013) Citing Websites. Quality Control Of Capsules. Retrieved date. 21st December 2013. From http://www.pharmainfo.net/quality-control-capsules

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